If you have 2007+, you could use conditional formatting as it has an option to highlight unique/duplicate values. This would be nice for data that already exists.
To handle data that will be entered in future, we can use data validation. Say you are dealing with cell A2. Data validation, custom rule is:
=COUNTIF($A:$IV,A2)=1
Then, go to the error tab and change the drop down from "stop" to just "information", and give a pertinent message, such as "Duplicate value entered". Apply this formatting to other cells as desired.