JagatDangi
As You should already noticed ...
There seems to be 'something' which do not work with that file (workbook)...
You've written that
with NEW workbook, You can do the graph - save - open - without any challenges.
If need to do it with 'something-wrong-file' ... it would need to do my term ... 'clear saving' ... I haven't find out how to do that!
( My term 'clear saving' is not same as 'save'.. 'saveas' ... 'save to other format' )
Sometimes it would help to open and save that file with other PC ... other version of Excel ... sometimes no help!
I've had something same cases ... those are tricky!
That's why ... it's wise to save files sometimes - close and open - to see that it works 'normally'.
(( This's like ... if You're running marathon (~42kms), it would be wise to check sometimes, that You're in correct road ))